In situ Structure of Rotavirus VP1 RNA-Dependent RNA Polymerase
نویسندگان
چکیده
منابع مشابه
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Double-stranded RNA (dsRNA) viruses transcribe and replicate RNA within an assembled, inner capsid particle; only plus-sense mRNA emerges into the intracellular milieu. During infectious entry of a rotavirus particle, the outer layer of its three-layer structure dissociates, delivering the inner double-layered particle (DLP) into the cytosol. DLP structures determined by X-ray crystallography a...
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Rotavirus single-shelled particles have several enzymatic activities that are involved with the synthesis of capped mRNAs both in vivo and in vitro. Because single-shelled particles must be structurally intact to carry out transcription, it has proven to be difficult to identify the protein within such particles that possesses associated RNA polymerase activity. One approach for characterizing ...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2019
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2019.06.016